Plasmid

Part:BBa_K3600006:Design

Designed by: yasmine koubaa   Group: iGEM20_EPFL   (2020-10-27)


prHSP12-yomScarlet-I-tADH1


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1184
    Illegal EcoRI site found at 1914
    Illegal XbaI site found at 1193
    Illegal XbaI site found at 1618
    Illegal XbaI site found at 2091
    Illegal SpeI site found at 3059
    Illegal PstI site found at 3068
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1184
    Illegal EcoRI site found at 1914
    Illegal SpeI site found at 3059
    Illegal PstI site found at 3068
    Illegal NotI site found at 1172
    Illegal NotI site found at 3078
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal suffix found in sequence at 2661
    Illegal EcoRI site found at 1184
    Illegal EcoRI site found at 1914
    Illegal BglII site found at 1636
    Illegal BglII site found at 2532
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1184
    Illegal EcoRI site found at 1914
    Illegal XbaI site found at 1193
    Illegal XbaI site found at 1618
    Illegal XbaI site found at 2091
    Illegal SpeI site found at 3059
    Illegal PstI site found at 3068
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1184
    Illegal EcoRI site found at 1914
    Illegal XbaI site found at 1193
    Illegal XbaI site found at 1618
    Illegal XbaI site found at 2091
    Illegal SpeI site found at 3059
    Illegal PstI site found at 3068
    Illegal NgoMIV site found at 3016
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

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Source

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References